阅微基因——专注多基因联检整体ag捕鱼平台的解决方案-1000炮捕鱼机

hot search
preservation solution extraction machine dna extraction kit blood card puncher 28a 23sp y prime plus rm-y 19x fragile x smn azf deafness folic acid msi ce cardiovascular drugs cyp2c19, cyp2c9, vkorc1

technology platform

microreader™ bovine 12/19 id system

microreader™ bovine 12/19 id system


introduction

microread™ bovine id system is designed for cattle parentage testing and identification using short tandem repeat (str) dna loci, which allows co-amplification of multiplex str loci in a single pcr reaction.


key features

■ simultaneous analysis of multiplex str markers in a single tube.
■selected markers, recommended by the international society of animal genetics (isag), national institute of standards and technology (nist) and food and agriculture organization of the united nations (fao).
■ high resistance to the presence of pcr inhibitors.
■ control dna provided for verification of pcr and electrophoresis conditions.
■ allele calling represents true alleles (no plus-a peaks).

the microread bovine id system

the microread bovine id system is offered for cattle parentage testing and identification using str loci. it has 2 versions.


■ the microread bovine 12 id system contains 12 widely used standard str markers recommended by the international society of animal genetics (isag), including: tgla227, bm2113, tgla53, eth10, sps115, tgla126, tgla122, inra23, bm1818, eth3, eth225, and bm1824.


■ the microread bovine 19 id system not only contains above 12 str markers, but also contain 7 more highly polymorphic str loci recommended by isag, nist and the food and agriculture organization of the united nations (fao), which is more informative, and it can provide higher discrimination in parentage testing and identification.


【applicable sample types】

the kit amplifies extracted dna from hair follicle, saliva swab and blood.

【provide control dna】
the kit includes bovine control dna for verification of acceptable pcr and electrophoresis conditions.

【no plus-a peaks】
reagents and protocols have been optimized to deliver similar peak sizes (amplification yields) for each loci. allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-a peaks' or 'split peaks' .

kit specifications

genotyping target

bovine genotyping and paternity testing

detection method

capillary electrophoresis

kit version

microread bovine 12 id system / microread bovine 19 id system

no. of reactions

100 reactions/ 200 reactions

for use with (application)

pcr

application of models

abi series sequencers, including 3730xl, 3500, 3130, seqstudio

storage

store at -20°c.

primer mix should be protected from light at all the times.